Subrate H2O2

Total control: 5 mL/run
H2O2- transparent solution, odorless, colourless
10% H2O2 (Trial 1)
H202-0.5mL
H20-4.5mL
Start gas volume: 100 mL
Stop gas volume: 99 mL
Time: 60sec/1 min
* Every trials time is the same
Observation- started to fizz, bubbles also started to appear, gas is being produced, and foam is also produced.

30% H202 (Trial 2)
H202-1.5mL
H20-3.5mL
Start gas volume: 99 mL
Stop gas volume: 95 mL
Time: 60 sec/1 min

50% H2O2 (Trial 3)
H202-2.5mL
H20-2.5mL
Start gas volume: 250 mL
Stop gas volume: 95 mL (MAX)
Time: 60 sec/1 min
*more bubbles and foam are seen

70% H2O2 (Trial 4)
H202-3.5mL
H20-1.5mL
Start gas volume: 310 mL
Stop gas volume: 150 mL
Time: 60 sec/1 min
*Foam-whitish/orange bubbles were produced. Reaction was much faster and quicker than the last one
* There were 2 trial runs to get this result. The lid was not sealed properly, which may have affected the result greatly

90% H2O2 (Trial 5)
H202-2.5mL
H20-2.5mL
Start gas volume: 250 mL
Stop gas volume: 100 mL
Time: 60 sec/1 min

Source of error-
The measurement for extracting H2O2 & H202 may not have been accurate.
The lid was not sealed on properly as oxygen may have interfered with the experiment
The timing may have been wrong.
The disc (liver) may have been less then 5 or more than 5

Subrate H2O2

Total control: 5 mL/run
H2O2- transparent solution, odorless, colourless
10% H2O2 (Trial 1)
H202-0.5mL
H20-4.5mL
Start gas volume: 100 mL
Stop gas volume: 99 mL
Time: 60sec/1 min
* Every trials time is the same
Observation- started to fizz, bubbles also started to appear, gas is being produced, and foam is also produced.

30% H202 (Trial 2)
H202-1.5mL
H20-3.5mL
Start gas volume: 99 mL
Stop gas volume: 95 mL
Time: 60 sec/1 min

50% H2O2 (Trial 3)
H202-2.5mL
H20-2.5mL
Start gas volume: 250 mL
Stop gas volume: 95 mL (MAX)
Time: 60 sec/1 min
*more bubbles and foam are seen

70% H2O2 (Trial 4)
H202-3.5mL
H20-1.5mL
Start gas volume: 310 mL
Stop gas volume: 150 mL
Time: 60 sec/1 min
*Foam-whitish/orange bubbles were produced. Reaction was much faster and quicker than the last one
* There were 2 trials run to get this result. The lid was not sealed properly, which may have affected the result greatly

90% H2O2 (Trial 5)
H202-2.5mL
H20-2.5mL
Start gas volume: 250 mL
Stop gas volume: 100 mL
Time: 60 sec/1 min

PCR vs Vector Cloning

Comparison of PCR Versus Gene Cloning

PCR-Polymerase Chain Reaction
The PCR is know to be a revolutionary technology.
(1) It is more efficient (as it needs much less amount of time to form the desired DNA; a single copy is enough)
(2) Does not require difficulty to store and costly restriction enzymes, ligase, and vector DNA, which reduces the cost drastically;
(3) PCR requires less work, time and skills needed to obtain desired DNA.
(4) Contains many more application then gene cloning. Typically, gene cloning experiments take 2-4 days, while PCR takes up to 4-5 hours. In addition,
(5) PCR is fully automated, while gene cloning is not. PCR does not require sequence information from the construction of primers and thermal cycler or PCR machine.
(6) PCR DOES NOT PRODUCE PROTEIN!
(7) Since PCR needs only one DNA segment of strands, if the strands is contaminated or replicated incompletely, the rest of the copies that are made will be damaged/corrupted as well.
8) Very useful during crimes and forensic investigation.

Vector Cloning
1) Takes a longer time to make the desired DNA
2) Requires DNA (EcoRI) and plasmid. Finding the gene of interest is needed also
3)Vector Cloning requires SPECIFIC RESTRICTION ENZYMES
4)The gene of interest is inserted into the plasmid in order to perform a recombinant DNA and produce desired protein.
5) Saves a lot more money.

Summarization of PCR vs Vector cloning
PCR is a considered to be a revolutionary technology. It is very efficient and saves a lot of times. It takes much less time to clone the desired DNA. Only a single copy of DNA is needed to make a desired copy of DNA
PCR saves more time and has many more useful applications than gene cloning.